去除高丰度蛋白质后水牛卵泡液的双向电泳差异分析

doi:10.11843/j.issn.0366-6964.2014.07.012

Abstract

Abstract:

This study employed swamp buffalo follicular fluids in Guangxi as the research object.First describes the purification of mature and immature buffalo follicular fluid samples using a ProteoExtract^TM Albumin/IgG Removal Kit，it then described the isolation of proteins from these purified mature and immature follicular fluids using the established and optimized 2DE system.Twenty-one different expression protein spots were found with Image Master 2D platinum software，10 of these proteins were up-regulated in immature buffalo follicular fluid，7 proteins were down-regulated，3 proteins deleted and 1 protein specifically expressed.Three proteins were successfully identified by mass spectrometry，they were probably sulfated glycoprotein-2，transferrin and HP protein.In conclusion，a group of different expression proteins from mature and immature buffalo follicular fluids were successfully isolated and identified to provide experimental basis for developmental microenviroment and maturation mechanism in buffalo oocytes.And transferrin played an important role in promoting the maturation of buffalo follicular fluid，while the maturation mechanism of other two proteins in buffalo follicular fluid was still not clear and it needs to be further studied.

CLC Number:

S823.8⁺3.2

HUANG Yu-lin，FU Qiang，HUANG De-lun，CHEN Fu-mei，HUANG Feng-ling，LIANG Xian-wei，ZHANG Ming，LU Ke-huan. 2-DE Differential Analysis of ALB/IgG Removal Buffalo Follicular Fluid[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1113-1119.

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2-DE Differential Analysis of ALB/IgG Removal Buffalo Follicular Fluid

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